anti dat Search Results


dat  (Alomone Labs)
91
Alomone Labs dat
FIGURE 7. Disruption of the OPPC domain affects protein segregation. PC12 cells that had been treated with NGF for 48 h were treated with 100 M RHC80267 in the bottom lane of A and F–J (blue in B–E) or with vehicle in the top lane of A and F–J (red in B–E) for 3 h. These cell preparations were analyzed as follows. A, in situ PLA1 activity assay by PED-A1 shown in green. B, remodeling assay with TMF from RHC80267-treated cells (blue) or from control cells (red). C, quantification of the remodeled products in B. The percentage of each remodeled product in TMF derived from RHC80267 treated cells (blue) or from control cells(red)isshown.D,RP-HPLCandMALDI-TOF-MSanalysisofTMFlipids(red,NGF;blue,NGFwithRHC80267).Thelipidsinfraction30oftheC8column(Fig. 2) of each sample were analyzed with MALDI-TOF-MS with 6.25 pmol of dimyristoylphosphatidylcholine as a calibration standard for quantification. E, OPPC content per protein in TMF (red, NGF; blue, NGF with RHC80267). Data are means from four independent experiments evaluated using two-tailed Student’s t test (*, p 0.02). Error bars, S.D. Immunofluorescent staining with mAb#15 (F; green), with antibody to Thy-I (G; green) and with antibody to <t>DAT</t> (H; red; double stainingthesamecellinG).I,dopamineuptakeassayusingafluorescentdopamineanalogshowningreen.J,immunofluorescentstainingwithantibodytoGo (green). The thick arrows and the thin arrows point to the presence and the absence of the activity, respectively, in each image. Bar, 40 m.
Dat, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dat  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology dat
FIGURE 7. Disruption of the OPPC domain affects protein segregation. PC12 cells that had been treated with NGF for 48 h were treated with 100 M RHC80267 in the bottom lane of A and F–J (blue in B–E) or with vehicle in the top lane of A and F–J (red in B–E) for 3 h. These cell preparations were analyzed as follows. A, in situ PLA1 activity assay by PED-A1 shown in green. B, remodeling assay with TMF from RHC80267-treated cells (blue) or from control cells (red). C, quantification of the remodeled products in B. The percentage of each remodeled product in TMF derived from RHC80267 treated cells (blue) or from control cells(red)isshown.D,RP-HPLCandMALDI-TOF-MSanalysisofTMFlipids(red,NGF;blue,NGFwithRHC80267).Thelipidsinfraction30oftheC8column(Fig. 2) of each sample were analyzed with MALDI-TOF-MS with 6.25 pmol of dimyristoylphosphatidylcholine as a calibration standard for quantification. E, OPPC content per protein in TMF (red, NGF; blue, NGF with RHC80267). Data are means from four independent experiments evaluated using two-tailed Student’s t test (*, p 0.02). Error bars, S.D. Immunofluorescent staining with mAb#15 (F; green), with antibody to Thy-I (G; green) and with antibody to <t>DAT</t> (H; red; double stainingthesamecellinG).I,dopamineuptakeassayusingafluorescentdopamineanalogshowningreen.J,immunofluorescentstainingwithantibodytoGo (green). The thick arrows and the thin arrows point to the presence and the absence of the activity, respectively, in each image. Bar, 40 m.
Dat, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit  (Proteintech)
95
Proteintech rabbit
FIGURE 7. Disruption of the OPPC domain affects protein segregation. PC12 cells that had been treated with NGF for 48 h were treated with 100 M RHC80267 in the bottom lane of A and F–J (blue in B–E) or with vehicle in the top lane of A and F–J (red in B–E) for 3 h. These cell preparations were analyzed as follows. A, in situ PLA1 activity assay by PED-A1 shown in green. B, remodeling assay with TMF from RHC80267-treated cells (blue) or from control cells (red). C, quantification of the remodeled products in B. The percentage of each remodeled product in TMF derived from RHC80267 treated cells (blue) or from control cells(red)isshown.D,RP-HPLCandMALDI-TOF-MSanalysisofTMFlipids(red,NGF;blue,NGFwithRHC80267).Thelipidsinfraction30oftheC8column(Fig. 2) of each sample were analyzed with MALDI-TOF-MS with 6.25 pmol of dimyristoylphosphatidylcholine as a calibration standard for quantification. E, OPPC content per protein in TMF (red, NGF; blue, NGF with RHC80267). Data are means from four independent experiments evaluated using two-tailed Student’s t test (*, p 0.02). Error bars, S.D. Immunofluorescent staining with mAb#15 (F; green), with antibody to Thy-I (G; green) and with antibody to <t>DAT</t> (H; red; double stainingthesamecellinG).I,dopamineuptakeassayusingafluorescentdopamineanalogshowningreen.J,immunofluorescentstainingwithantibodytoGo (green). The thick arrows and the thin arrows point to the presence and the absence of the activity, respectively, in each image. Bar, 40 m.
Rabbit, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti slc6a3 dat  (Novus Biologicals)
93
Novus Biologicals anti slc6a3 dat
FIGURE 7. Disruption of the OPPC domain affects protein segregation. PC12 cells that had been treated with NGF for 48 h were treated with 100 M RHC80267 in the bottom lane of A and F–J (blue in B–E) or with vehicle in the top lane of A and F–J (red in B–E) for 3 h. These cell preparations were analyzed as follows. A, in situ PLA1 activity assay by PED-A1 shown in green. B, remodeling assay with TMF from RHC80267-treated cells (blue) or from control cells (red). C, quantification of the remodeled products in B. The percentage of each remodeled product in TMF derived from RHC80267 treated cells (blue) or from control cells(red)isshown.D,RP-HPLCandMALDI-TOF-MSanalysisofTMFlipids(red,NGF;blue,NGFwithRHC80267).Thelipidsinfraction30oftheC8column(Fig. 2) of each sample were analyzed with MALDI-TOF-MS with 6.25 pmol of dimyristoylphosphatidylcholine as a calibration standard for quantification. E, OPPC content per protein in TMF (red, NGF; blue, NGF with RHC80267). Data are means from four independent experiments evaluated using two-tailed Student’s t test (*, p 0.02). Error bars, S.D. Immunofluorescent staining with mAb#15 (F; green), with antibody to Thy-I (G; green) and with antibody to <t>DAT</t> (H; red; double stainingthesamecellinG).I,dopamineuptakeassayusingafluorescentdopamineanalogshowningreen.J,immunofluorescentstainingwithantibodytoGo (green). The thick arrows and the thin arrows point to the presence and the absence of the activity, respectively, in each image. Bar, 40 m.
Anti Slc6a3 Dat, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti dat  (Novus Biologicals)
94
Novus Biologicals mouse anti dat
FIGURE 7. Disruption of the OPPC domain affects protein segregation. PC12 cells that had been treated with NGF for 48 h were treated with 100 M RHC80267 in the bottom lane of A and F–J (blue in B–E) or with vehicle in the top lane of A and F–J (red in B–E) for 3 h. These cell preparations were analyzed as follows. A, in situ PLA1 activity assay by PED-A1 shown in green. B, remodeling assay with TMF from RHC80267-treated cells (blue) or from control cells (red). C, quantification of the remodeled products in B. The percentage of each remodeled product in TMF derived from RHC80267 treated cells (blue) or from control cells(red)isshown.D,RP-HPLCandMALDI-TOF-MSanalysisofTMFlipids(red,NGF;blue,NGFwithRHC80267).Thelipidsinfraction30oftheC8column(Fig. 2) of each sample were analyzed with MALDI-TOF-MS with 6.25 pmol of dimyristoylphosphatidylcholine as a calibration standard for quantification. E, OPPC content per protein in TMF (red, NGF; blue, NGF with RHC80267). Data are means from four independent experiments evaluated using two-tailed Student’s t test (*, p 0.02). Error bars, S.D. Immunofluorescent staining with mAb#15 (F; green), with antibody to Thy-I (G; green) and with antibody to <t>DAT</t> (H; red; double stainingthesamecellinG).I,dopamineuptakeassayusingafluorescentdopamineanalogshowningreen.J,immunofluorescentstainingwithantibodytoGo (green). The thick arrows and the thin arrows point to the presence and the absence of the activity, respectively, in each image. Bar, 40 m.
Mouse Anti Dat, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti dat  (Atlas Antibodies)
93
Atlas Antibodies mouse anti dat
FIGURE 7. Disruption of the OPPC domain affects protein segregation. PC12 cells that had been treated with NGF for 48 h were treated with 100 M RHC80267 in the bottom lane of A and F–J (blue in B–E) or with vehicle in the top lane of A and F–J (red in B–E) for 3 h. These cell preparations were analyzed as follows. A, in situ PLA1 activity assay by PED-A1 shown in green. B, remodeling assay with TMF from RHC80267-treated cells (blue) or from control cells (red). C, quantification of the remodeled products in B. The percentage of each remodeled product in TMF derived from RHC80267 treated cells (blue) or from control cells(red)isshown.D,RP-HPLCandMALDI-TOF-MSanalysisofTMFlipids(red,NGF;blue,NGFwithRHC80267).Thelipidsinfraction30oftheC8column(Fig. 2) of each sample were analyzed with MALDI-TOF-MS with 6.25 pmol of dimyristoylphosphatidylcholine as a calibration standard for quantification. E, OPPC content per protein in TMF (red, NGF; blue, NGF with RHC80267). Data are means from four independent experiments evaluated using two-tailed Student’s t test (*, p 0.02). Error bars, S.D. Immunofluorescent staining with mAb#15 (F; green), with antibody to Thy-I (G; green) and with antibody to <t>DAT</t> (H; red; double stainingthesamecellinG).I,dopamineuptakeassayusingafluorescentdopamineanalogshowningreen.J,immunofluorescentstainingwithantibodytoGo (green). The thick arrows and the thin arrows point to the presence and the absence of the activity, respectively, in each image. Bar, 40 m.
Mouse Anti Dat, supplied by Atlas Antibodies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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da transporter blockers  (MedChemExpress)
94
MedChemExpress da transporter blockers
FIGURE 7. Disruption of the OPPC domain affects protein segregation. PC12 cells that had been treated with NGF for 48 h were treated with 100 M RHC80267 in the bottom lane of A and F–J (blue in B–E) or with vehicle in the top lane of A and F–J (red in B–E) for 3 h. These cell preparations were analyzed as follows. A, in situ PLA1 activity assay by PED-A1 shown in green. B, remodeling assay with TMF from RHC80267-treated cells (blue) or from control cells (red). C, quantification of the remodeled products in B. The percentage of each remodeled product in TMF derived from RHC80267 treated cells (blue) or from control cells(red)isshown.D,RP-HPLCandMALDI-TOF-MSanalysisofTMFlipids(red,NGF;blue,NGFwithRHC80267).Thelipidsinfraction30oftheC8column(Fig. 2) of each sample were analyzed with MALDI-TOF-MS with 6.25 pmol of dimyristoylphosphatidylcholine as a calibration standard for quantification. E, OPPC content per protein in TMF (red, NGF; blue, NGF with RHC80267). Data are means from four independent experiments evaluated using two-tailed Student’s t test (*, p 0.02). Error bars, S.D. Immunofluorescent staining with mAb#15 (F; green), with antibody to Thy-I (G; green) and with antibody to <t>DAT</t> (H; red; double stainingthesamecellinG).I,dopamineuptakeassayusingafluorescentdopamineanalogshowningreen.J,immunofluorescentstainingwithantibodytoGo (green). The thick arrows and the thin arrows point to the presence and the absence of the activity, respectively, in each image. Bar, 40 m.
Da Transporter Blockers, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dat antibody mab369  (Merck KGaA)
90
Merck KGaA dat antibody mab369
FIGURE 7. Disruption of the OPPC domain affects protein segregation. PC12 cells that had been treated with NGF for 48 h were treated with 100 M RHC80267 in the bottom lane of A and F–J (blue in B–E) or with vehicle in the top lane of A and F–J (red in B–E) for 3 h. These cell preparations were analyzed as follows. A, in situ PLA1 activity assay by PED-A1 shown in green. B, remodeling assay with TMF from RHC80267-treated cells (blue) or from control cells (red). C, quantification of the remodeled products in B. The percentage of each remodeled product in TMF derived from RHC80267 treated cells (blue) or from control cells(red)isshown.D,RP-HPLCandMALDI-TOF-MSanalysisofTMFlipids(red,NGF;blue,NGFwithRHC80267).Thelipidsinfraction30oftheC8column(Fig. 2) of each sample were analyzed with MALDI-TOF-MS with 6.25 pmol of dimyristoylphosphatidylcholine as a calibration standard for quantification. E, OPPC content per protein in TMF (red, NGF; blue, NGF with RHC80267). Data are means from four independent experiments evaluated using two-tailed Student’s t test (*, p 0.02). Error bars, S.D. Immunofluorescent staining with mAb#15 (F; green), with antibody to Thy-I (G; green) and with antibody to <t>DAT</t> (H; red; double stainingthesamecellinG).I,dopamineuptakeassayusingafluorescentdopamineanalogshowningreen.J,immunofluorescentstainingwithantibodytoGo (green). The thick arrows and the thin arrows point to the presence and the absence of the activity, respectively, in each image. Bar, 40 m.
Dat Antibody Mab369, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-dat primary antibody  (Merck KGaA)
90
Merck KGaA anti-dat primary antibody
FIGURE 7. Disruption of the OPPC domain affects protein segregation. PC12 cells that had been treated with NGF for 48 h were treated with 100 M RHC80267 in the bottom lane of A and F–J (blue in B–E) or with vehicle in the top lane of A and F–J (red in B–E) for 3 h. These cell preparations were analyzed as follows. A, in situ PLA1 activity assay by PED-A1 shown in green. B, remodeling assay with TMF from RHC80267-treated cells (blue) or from control cells (red). C, quantification of the remodeled products in B. The percentage of each remodeled product in TMF derived from RHC80267 treated cells (blue) or from control cells(red)isshown.D,RP-HPLCandMALDI-TOF-MSanalysisofTMFlipids(red,NGF;blue,NGFwithRHC80267).Thelipidsinfraction30oftheC8column(Fig. 2) of each sample were analyzed with MALDI-TOF-MS with 6.25 pmol of dimyristoylphosphatidylcholine as a calibration standard for quantification. E, OPPC content per protein in TMF (red, NGF; blue, NGF with RHC80267). Data are means from four independent experiments evaluated using two-tailed Student’s t test (*, p 0.02). Error bars, S.D. Immunofluorescent staining with mAb#15 (F; green), with antibody to Thy-I (G; green) and with antibody to <t>DAT</t> (H; red; double stainingthesamecellinG).I,dopamineuptakeassayusingafluorescentdopamineanalogshowningreen.J,immunofluorescentstainingwithantibodytoGo (green). The thick arrows and the thin arrows point to the presence and the absence of the activity, respectively, in each image. Bar, 40 m.
Anti Dat Primary Antibody, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary antibody dat sc biotech rabbit polyclonal igg anti-dat (h-80) sc-14002  (biotechrabbit)
90
biotechrabbit primary antibody dat sc biotech rabbit polyclonal igg anti-dat (h-80) sc-14002
FIGURE 7. Disruption of the OPPC domain affects protein segregation. PC12 cells that had been treated with NGF for 48 h were treated with 100 M RHC80267 in the bottom lane of A and F–J (blue in B–E) or with vehicle in the top lane of A and F–J (red in B–E) for 3 h. These cell preparations were analyzed as follows. A, in situ PLA1 activity assay by PED-A1 shown in green. B, remodeling assay with TMF from RHC80267-treated cells (blue) or from control cells (red). C, quantification of the remodeled products in B. The percentage of each remodeled product in TMF derived from RHC80267 treated cells (blue) or from control cells(red)isshown.D,RP-HPLCandMALDI-TOF-MSanalysisofTMFlipids(red,NGF;blue,NGFwithRHC80267).Thelipidsinfraction30oftheC8column(Fig. 2) of each sample were analyzed with MALDI-TOF-MS with 6.25 pmol of dimyristoylphosphatidylcholine as a calibration standard for quantification. E, OPPC content per protein in TMF (red, NGF; blue, NGF with RHC80267). Data are means from four independent experiments evaluated using two-tailed Student’s t test (*, p 0.02). Error bars, S.D. Immunofluorescent staining with mAb#15 (F; green), with antibody to Thy-I (G; green) and with antibody to <t>DAT</t> (H; red; double stainingthesamecellinG).I,dopamineuptakeassayusingafluorescentdopamineanalogshowningreen.J,immunofluorescentstainingwithantibodytoGo (green). The thick arrows and the thin arrows point to the presence and the absence of the activity, respectively, in each image. Bar, 40 m.
Primary Antibody Dat Sc Biotech Rabbit Polyclonal Igg Anti Dat (H 80) Sc 14002, supplied by biotechrabbit, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-dat antibody  (Merck KGaA)
90
Merck KGaA anti-dat antibody
FIGURE 7. Disruption of the OPPC domain affects protein segregation. PC12 cells that had been treated with NGF for 48 h were treated with 100 M RHC80267 in the bottom lane of A and F–J (blue in B–E) or with vehicle in the top lane of A and F–J (red in B–E) for 3 h. These cell preparations were analyzed as follows. A, in situ PLA1 activity assay by PED-A1 shown in green. B, remodeling assay with TMF from RHC80267-treated cells (blue) or from control cells (red). C, quantification of the remodeled products in B. The percentage of each remodeled product in TMF derived from RHC80267 treated cells (blue) or from control cells(red)isshown.D,RP-HPLCandMALDI-TOF-MSanalysisofTMFlipids(red,NGF;blue,NGFwithRHC80267).Thelipidsinfraction30oftheC8column(Fig. 2) of each sample were analyzed with MALDI-TOF-MS with 6.25 pmol of dimyristoylphosphatidylcholine as a calibration standard for quantification. E, OPPC content per protein in TMF (red, NGF; blue, NGF with RHC80267). Data are means from four independent experiments evaluated using two-tailed Student’s t test (*, p 0.02). Error bars, S.D. Immunofluorescent staining with mAb#15 (F; green), with antibody to Thy-I (G; green) and with antibody to <t>DAT</t> (H; red; double stainingthesamecellinG).I,dopamineuptakeassayusingafluorescentdopamineanalogshowningreen.J,immunofluorescentstainingwithantibodytoGo (green). The thick arrows and the thin arrows point to the presence and the absence of the activity, respectively, in each image. Bar, 40 m.
Anti Dat Antibody, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-nis antibody slc6a3  (MyBiosource Biotechnology)
90
MyBiosource Biotechnology anti-nis antibody slc6a3
FIGURE 7. Disruption of the OPPC domain affects protein segregation. PC12 cells that had been treated with NGF for 48 h were treated with 100 M RHC80267 in the bottom lane of A and F–J (blue in B–E) or with vehicle in the top lane of A and F–J (red in B–E) for 3 h. These cell preparations were analyzed as follows. A, in situ PLA1 activity assay by PED-A1 shown in green. B, remodeling assay with TMF from RHC80267-treated cells (blue) or from control cells (red). C, quantification of the remodeled products in B. The percentage of each remodeled product in TMF derived from RHC80267 treated cells (blue) or from control cells(red)isshown.D,RP-HPLCandMALDI-TOF-MSanalysisofTMFlipids(red,NGF;blue,NGFwithRHC80267).Thelipidsinfraction30oftheC8column(Fig. 2) of each sample were analyzed with MALDI-TOF-MS with 6.25 pmol of dimyristoylphosphatidylcholine as a calibration standard for quantification. E, OPPC content per protein in TMF (red, NGF; blue, NGF with RHC80267). Data are means from four independent experiments evaluated using two-tailed Student’s t test (*, p 0.02). Error bars, S.D. Immunofluorescent staining with mAb#15 (F; green), with antibody to Thy-I (G; green) and with antibody to <t>DAT</t> (H; red; double stainingthesamecellinG).I,dopamineuptakeassayusingafluorescentdopamineanalogshowningreen.J,immunofluorescentstainingwithantibodytoGo (green). The thick arrows and the thin arrows point to the presence and the absence of the activity, respectively, in each image. Bar, 40 m.
Anti Nis Antibody Slc6a3, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIGURE 7. Disruption of the OPPC domain affects protein segregation. PC12 cells that had been treated with NGF for 48 h were treated with 100 M RHC80267 in the bottom lane of A and F–J (blue in B–E) or with vehicle in the top lane of A and F–J (red in B–E) for 3 h. These cell preparations were analyzed as follows. A, in situ PLA1 activity assay by PED-A1 shown in green. B, remodeling assay with TMF from RHC80267-treated cells (blue) or from control cells (red). C, quantification of the remodeled products in B. The percentage of each remodeled product in TMF derived from RHC80267 treated cells (blue) or from control cells(red)isshown.D,RP-HPLCandMALDI-TOF-MSanalysisofTMFlipids(red,NGF;blue,NGFwithRHC80267).Thelipidsinfraction30oftheC8column(Fig. 2) of each sample were analyzed with MALDI-TOF-MS with 6.25 pmol of dimyristoylphosphatidylcholine as a calibration standard for quantification. E, OPPC content per protein in TMF (red, NGF; blue, NGF with RHC80267). Data are means from four independent experiments evaluated using two-tailed Student’s t test (*, p 0.02). Error bars, S.D. Immunofluorescent staining with mAb#15 (F; green), with antibody to Thy-I (G; green) and with antibody to DAT (H; red; double stainingthesamecellinG).I,dopamineuptakeassayusingafluorescentdopamineanalogshowningreen.J,immunofluorescentstainingwithantibodytoGo (green). The thick arrows and the thin arrows point to the presence and the absence of the activity, respectively, in each image. Bar, 40 m.

Journal: Journal of Biological Chemistry

Article Title: Functional Compartmentalization of the Plasma Membrane of Neurons by a Unique Acyl Chain Composition of Phospholipids

doi: 10.1074/jbc.m114.571075

Figure Lengend Snippet: FIGURE 7. Disruption of the OPPC domain affects protein segregation. PC12 cells that had been treated with NGF for 48 h were treated with 100 M RHC80267 in the bottom lane of A and F–J (blue in B–E) or with vehicle in the top lane of A and F–J (red in B–E) for 3 h. These cell preparations were analyzed as follows. A, in situ PLA1 activity assay by PED-A1 shown in green. B, remodeling assay with TMF from RHC80267-treated cells (blue) or from control cells (red). C, quantification of the remodeled products in B. The percentage of each remodeled product in TMF derived from RHC80267 treated cells (blue) or from control cells(red)isshown.D,RP-HPLCandMALDI-TOF-MSanalysisofTMFlipids(red,NGF;blue,NGFwithRHC80267).Thelipidsinfraction30oftheC8column(Fig. 2) of each sample were analyzed with MALDI-TOF-MS with 6.25 pmol of dimyristoylphosphatidylcholine as a calibration standard for quantification. E, OPPC content per protein in TMF (red, NGF; blue, NGF with RHC80267). Data are means from four independent experiments evaluated using two-tailed Student’s t test (*, p 0.02). Error bars, S.D. Immunofluorescent staining with mAb#15 (F; green), with antibody to Thy-I (G; green) and with antibody to DAT (H; red; double stainingthesamecellinG).I,dopamineuptakeassayusingafluorescentdopamineanalogshowningreen.J,immunofluorescentstainingwithantibodytoGo (green). The thick arrows and the thin arrows point to the presence and the absence of the activity, respectively, in each image. Bar, 40 m.

Article Snippet: Antibodies to Thy-1 (Millipore, MAB1406) and to DAT (Alomone Labs, AMT-003) were used in 100-fold dilution in combination with 5000-fold diluted Alexa 555-labeled antibody to rabbit IgG and Alexa 488-labeled antibody to mouse IgG.

Techniques: Disruption, In Situ, Activity Assay, Control, Derivative Assay, Two Tailed Test, Staining